Chromolith® RP-18 endcapped HighResolution
The faster way to trouble-free high resolution separations
Revolutionary monolithic silica columns are a single rod of high purity monolithic silica instead of particles. A unique bimodal pore structure gives greatly improved chromatographic performance. This leads mainly to improved separation efficiency and column back-pressure and allows for faster flow rates, considerably reducing the analysis time while enhancing the lifetime.
Benefits of the new Chromolith® HighResolution
- Column performance corresponds to sub 3 µm particle-packed columns and is at least 50% higher compared with our standard Chromolith® columns
- Back-pressure at least half that of particular columns
- Minimum 30% longer column lifetime compared with particle-packed columns
The new Chromolith® HighResolution columns offer higher efficiency and improved peak shape combined with lower back-pressure which is more than two times lower than any particulate packed column with the same dimensions.
Differentiation between Chromolith® and Chromolith® HighResolution
The new Chromolith® HighResolution has at least 50% higher efficiency, excellent peak symmetry and still a more than 30% longer lifetime compared with particulate columns. Two Chromolith® HighResolution columns can easily be coupled in order to achieve even higher resolution. A endcapped stationary phase enables the elution of basic compounds with no peak tailing.
Matrix-rich samples should be analysed with Chromolith® as this type of column will have a longer lifetime. In addition, a lower back-pressure would allow coupling of even more columns if necessary.
| Chromolith® Performance RP-18e, 100-4.6 nm | Chromolith® HighResolution RP-18e, 100-4.6 nm |
||
|---|---|---|---|
| Mobile phase | Acetonitrile/water 60/40 |  |
 |
| Flow rate | 2.0 ml/min | ||
| Detection | UV 254 nm | ||
| Temperature | ambient | ||
| Inj. volume | 5 µl | ||
| Sample | 1. Urea 2. Biphenyl-2-ol 3. Progesterone 4. Hexanophenone 5. Anthracene |
Specification of Chromolith® HighResolution RP-18 endcapped
| Silica type | High-purity |
| Particle size | Monolithic |
| Macropore size | 1.15 micrometer |
| Mesopore size | 15 nm (150 Angström) |
| Pore volume | 1 mL/g |
| Total pore volume | 2.9 mL/g |
| Surface area | 250 m2/g |
| Surface modification | RP-18 endcapped |
| Carbon content | 18 % |
Method transfer from particulate to monolithic Chromolith® columns
Method transfer from particulate to monolithic Chromolith® columns is made easy since the chromatographic selectivity of Chromolith® columns is very similar to that of many modern particulate HPLC columns (e.g. Purospher®). Chromolith® columns are now included in the US Pharmacopoeia in categories L1, L3 and L7.
Further suggested reading
